Injectoporation
WebbHere you will find our spare parts, our tools and equipment for the repairing of Diesel Injectors, Diesel pumps and Turbochargers, the exploded drawings, all Redat products and their applications. In our Diesel catalogues you can find: Denso, Caterpillar Perkins, Bosch, MTU L’Orange, Delphi, Cummins, Siemens and Unit injector. WebbInjectoporation was performed as previously described (Du et al., 2024). Briefly, the cochlear sensory epithelia were isolated from P2 mice and cultured in DMEM/F12 with 1.5 μg/ml ampicillin. Expression plasmids (0.2 μg/μl in Hanks’ balanced salt solution) were delivered to hair cells using a glass pipette of 2 μm tip diameter.
Injectoporation
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WebbTo investigate whether GRXCR2 (Δ36–95) is also concentrated at the base of stereocilia, injectoporation (Xiong et al., 2014) was performed to express Myc-tagged GRXCR2 … Webb29 mars 2024 · Culture and injectoporation of cochlear explants. Injectoporation of cochlear explants was performed following recently established protocols (Xiong et al., …
WebbGale Academic OneFile includes Using injectoporation to deliver genes to mechanosensor by Wei Xiong, Thomas Wagner, Linxuan Yan, . Click to explore. Webb8 juli 2024 · Mechanotransduction currents were recorded one day after injectoporation by stimulating hair bundles of OHCs with a stiff glass probe. Because of variability in bundle shape following injectoporation, a probe with approximate bundle size was lowered onto the bundle from the top.
WebbRe-imagining discovery and access to research: grants, datasets, publications, citations, clinical trials, patents and policy documents in one place. Webb29 mars 2024 · Culture and injectoporation of cochlear explants. Injectoporation of cochlear explants was performed following recently established protocols (Xiong et al., 2014). In brief, cochleae were dissected from postnatal (P) day 4 and P5 wild-type mice, cut into three pieces, and cultured for 4 h in DMEM/F12 medium with 10 ng/μl ampicillin.
Webb13 nov. 2024 · Take advantage of our tissue injectoporation system, 121 we electroporated m-3j-gRNA1 and wildtype spCas9 (PX330-m-3j-gRNA1) in 122 postnatal day 3 av3j/av3j cochleae and cultured for 2 days in vitro (P3+2DIV). 123 PCDH15 puncta was specifically detected in some electroporated av3j/av3j
WebbInjectoporation in inner ear. Research article PDZD7-MYO7A complex identified in enriched stereocilia membranes Clive P Morgan et al. DOI: 10.7554/eLife.18312 Your … inkbird water thermometerhttp://www.auditory-neuroscience.uni-goettingen.de/group_vogl.html mobile patrol mugshots buffalo nyWebbFigure 3 Diagram of the microinjection procedure and evaluation of the injectoporation efficiency. (a) The diagram shows a cross-section of the organ of Corti. (b) For gene transfer into OHCs, the microinjection pipette penetrates the surface between the second and third row of OHCs (top); to transfect IHCs, the microinjection pipette penetrates the … mobile pathways llcWebb15 maj 2024 · Using injectoporation, we expressed the cDNAs in OHCs from Tomt add/add mice at P3 and analyzed transducer currents after one day in culture (Figure 7O–Q). OHCs from the mid-apical region of the cochlea expressing the cDNAs were identified by GFP fluorescence and mechanotransduction currents were evaluated by … mobile patrol cortland countyWebbInjectoporation of Ripor2-constructs and immunostaining The generation of Ripor2 LacZ/LacZ mice has been described previously. 19 For Ripor2 DNA construct generation, Ripor2 cDNA (NM_029679.2, without exon 13) was amplified from a mouse-cochlear cDNA library and cloned into a pEGFP-N3-derived vector from which the EGFP (enhanced … inkbird wifi home assistantWebb18 sep. 2014 · A method termed injectoporation is described that combines tissue microinjection with electroporation to express cDNAs and shRNAs in mouse … mobile patrol inmate lookup michiganWebbscientific article inkbird wifi gateway