SpletRestriction Enzymes BbsI. A restriction enzyme or restriction endonuclease is defined as a protein that recognizes a specific, short nucleotide sequence and cuts the DNA only at or … SpletI am trying to prepare a construct using pCFD6 plasmid as the vector and 2 overlapping sequences as the insert. As per the protocol, I performed the Gibson assembly reaction in …

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SpletWe thank F. Port and S. Bullock for sharing the UASt-sgRNA plasmid (pCFD6) prior to publication, H.-W. Tang for assistance generating FLP-out CRISPRa stocks, and R. Binari … Splet01. jan. 2024 · As the Dro5 genes lie in two clusters—Dro5A (containing four genes) and Dro5B (containing three genes; Fig. 2A)—separated by four non-Dro5 EcKLs, we used an … fried pineapple chicken

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http://www.crisprflydesign.org/plasmids/ Splet这一篇我们要为大家讲解的就是 抗性基因（resistant gene） 在质粒图谱中的AmpR、KanR等表示质粒载体中的筛选标签，多为抗生素抗性基因，方便后续通过抗生素筛选阳 … http://www.biovector.net/product/319200.html fried pineapple pie